Extracellular matrix affects mesangial cell phenotype
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Extracellular matrix affects mesangial cell phenotype effect of collagen type I and heparin on mesangial cell proliferation and contractility. by Tihomir Miralem

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Published .
Written in English


Book details:

The Physical Object
Pagination295 leaves.
Number of Pages295
ID Numbers
Open LibraryOL19986753M
ISBN 100612414760

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  Further the cells expressed increased levels of mRNA for extracellular matrix (ECM) proteins (COL1A1, COL1A2, COL4A1 and LAMB1), matrix metalloproteinase enzymes (MMP9) and tissue inhibitors of matrix metalloproteinases (TIMP1). Treatment of MCs with serum from patients with active LN was able to induce a similar, albeit milder by: 2. In this study, cell migration assays are performed with different types of extracellular matrix on smooth muscle cells. Cells were seeded with DMEM + 10% FBS in tissue culture dishes where a polydimethylsiloxane (PDMS)-made block was placed in the center of the : T. Ohashi, Y. Hagiwara.   Cell density can vary greatly between different tissues of an animal, from tightly-packed muscle cells with many direct cell-to-cell contacts to liver tissue, in which some of the cells are only loosely organized, suspended in a web of extracellular matrix. Figure \(\PageIndex{1}\). Extracellular matrix . Mesangial cell proliferation and their dysfunction resulting from glomerulonephritis contribute to extracellular matrix degradation and remodeling and ultimately to glomerular fibrosis. Mesangial expansion and the degree of fibrosis present correlate inversely with glomerular filtration rate.

Ohyama K, Seyer JM, Raghow R, Kang AH. Extracellular matrix phenotype of rat mesangial cells in culture. Biosynthesis of collagen types I, III, IV, and V and a low molecular weight collagenous component and their regulation by dexamethasone. J Lab Clin Med. Aug; (2)–   Ayo SH, Radnik RA, Glass WF, 2nd, Garoni JA, Rampt ER, Appling DR. Increased extracellular matrix synthesis and mRNA in mesangial cells grown in high-glucose medium. Am J Physiol. ; F–F Beccerica E, Ferretti G, Curatola G, Cervini C. Diacetylrhein and rhein: in vivo and in vitro effect on lymphocyte membrane fluidity. Syndecans as receptors and organizers of the extracellular matrix. Cell Tissue Res. –46 ; Xiong J, Wang Y, Zhu Z, Liu J, Wang Y, Zhang C, Hammes H-P, Lang F, Feng Y. NG2 proteoglycan increases mesangial cell proliferation and extracellular matrix production. Biochem Biophys Res Commun. –   The results support the concept that the biochemical signals in nonmalignant ECM can downregulate neoplastic cell phenotype with minimal, and sometimes opposite, effects on normal cells. PI3K-Akt signaling has been implicated in EAC progression and these ECM-mediated effects may be favorable for an esophageal therapy following cancer resection.

  This triggers a number of events resulting in mesangial expansion with increased extracellular matrix of abnormal composition (rich in tenascin), often forming mesangial nodules replacing the predominant collagen IV matrix that is present in the normal mesangium, 17, 18, Under the pathologic conditions, mesangial cells are activated, leading to hyperproliferation and excess extracellular matrix (ECM). Moreover, mesangial cells also secrete several kinds of inflammatory cytokines, adhesion molecules, chemokines, and enzymes, all of which participate in the process of renal glomerular fibrosis. Diabetic nephropathy is characterized by an accumulation of extracellular matrix (ECM) components caused by an imbalance between synthesis and degradation (5,6). Mesangial cells (MC) have been postulated to be a major contributor to the sclerotic lesion in glomeruli of . Mesangial cell behavior in a three-dimensional extracellular matrix. To understand the role of mesangial matrix in regulating responses of mesangial cells (MCs), particularly as a cause of disease, we examined the behavior of MCs cultured in a three-dimensional extracellular matrix (ECM). Mouse and rat MCs were incorporated into ECM.